Cell Biologics/ZDF Rat Diabetic Artery Fibroblasts/RD-6073/ T75 Flask

ZDFRatDiabeticArteryFibroblasts

CatalogNo. RD-6073

SuggestedMedium: FibroblastMedium/wKit–500ml

CatalogNo. M2267

ProductDescription

ZDFRatDiabeticArteryFibroblastsfrom CellBIOLOGics areisolatedfromarterytissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶ cellspermlandisdeliveredfrozen.

ProductTesting

ZDFRatDiabetic Artery Fibroblasts from CellBiologics aretestedfornegativeexpressionofvonWillebrandFactorExpression/FactorVIII,cytokeratin18,andalphasmoothmuscleactin.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

ZDFRatDiabetic Artery Fibroblasts canbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

Storageof CellBiologics Products

CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest. Primarycellscanneverbekeptat-20°C.

AuthorizedUsesof CellBiologics Products

ZDFRatDiabetic Artery Fibroblasts from CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,for invitro diagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.

WarrantyandLiABIlity

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.