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CellnTec/CnT-PR-H/CnT-PR-H/1 Ea

wound-closure_cnt-pr-h

Keratinocytesinascratchassayshow5xgreaterresponsivenesstothepositivecontroltreatmentwhengrowinginCnT-PR-Hhomeostasismediumcomparedwiththestandardproliferationmedium.

TheCnT-PR-Hmediumisdesignedformaintenanceofprimaryhumankeratinocytesinahomeostaticstatewithonlyminimalgrowthfactorstimulii,sufficienttomaintaintheirfunctionforuptoaweekinculture.

Itcaneasilybeupgradedforclinicalapplications.Contactusatscientist@CellnTec.comforfurtherdetailsandpricing.

Intheabsenceofthestronggrowthfactorstimulationfoundinstandardproliferationmedia,keratinocytesgrowinginCnT-PR-Hshowheightedresponsivenesstoexperimentalstimulii(suchasactiveingredientstostimulatewoundclosure,asshownatleft).

Requestyourtestsampletoday!


CnT-PrimeKeratinocyteHomeostasisMedium

CnT-PR-Hisafullysupplemented,frozen,andreadytouseculturemedium,designedformaintenanceofprimarykeratinocytesunahomeostaticstateforuptooneweek.Itdoesnotcontainantibiotics/antimycotics.

Species
Developedforhumankeratinocytes.Maybeusedforotherspeciesaswell.
Tissuetype
Epithelia
Packsize
250mLbottle,fullysupplementedwithgrowthfactors.Nofurtheradditionsrequired.
Productuse
Developedformaintenanceofprimarykeratinocytesinahomoestaticstateforupto1weekofculture.
MediaType
2D-Prolif
Defined
Yes
ACF
No
Culturecondition
Thismediumisdesignedforuseina5%CO2atmosphere.Forsuggestedcellcultureprotocols,pleaseseeourresourcessection.
Note
Forroutinecellcultivation,CELLnTECrecommendstoworkwithoutantibiotics/antimycotics.
Storage/Shelflife
Storefrozenbelow-15oC.Forbestbeforedate,seelabel.Topreparemediumforuse,itispreferabletothawovernightat4oC.Alternatively,mediummaybethawedatroomtemperatureforapprox5-6hours.Ifthawingatroomtemperature,ensurecontentsdonotriseabove15oCattheendofthethawingprocess.Donotthawinahotwaterbath.Oncethawed,mediumhasaremainingshelf-lifeof6-weekswhenstoredat4oCinthedark.Certainculturemediacomponentsareverysensitivetolight.Minimizelightexposureatalltimes.
Qualitycontrol
Mediafunctionalityandcellmorphologytestedbycellcultivationwithprimaryhumankeratinocytes.Osmolality,pHandconcentrationofvariousionsaretested.Freeofbacteria,fungiandmycoplasmacontamination.
Shippingcondition
CnT-PR-Hisshippedfrozen.
Intendeduse
Forresearchuseonly.Notforuseintherapyordiagnostics.
Lastupdate
2017-09-27
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