TheHiSeqRapidDuocBotSampleLoADIngKitallowsmaximumindexingfreedominrapidrunmodebyenablingindividuallyaddressablelanes.ThiskitisanoptionalaccessoryforHiSeqRapidClusterkits(whichrequireacBotinstrument),andisdesignedforuseintherapidrunworkflowofHiSeq2500and1500sequencers.
TheHiSeqRapidDuocBotSampleLoadingKitallowseachrapidflowcelllanetobeloadedwithauniquesampletemplatepool.TheduokitaccomplishesindividuallaneloadingbyallowingacBotinstrumenttobeusedforthetemplatehybridizationandfirstextensionstepsoftheclustergenerationprocess.TousethecBottoload2librariesontoarapidflowcell,1HiSeqRapidDuocBotSampleLoadingKitisrequired.Eachkitcontainssufficientreagentsforloading1flowcell.
Aspecificallydesignedduomanifolddirectseachofthe2samplesintothe2separaterapidflowcelllanes.ThecBotportiontakesaboutanhour,afterwhichtheflowcellismovedtotheHiSeq2500or1500forfurtherclustergenerationstepsusingaHiSeqRapidClusterKit.
TheHiSeqRapidDuocBotSampleLoadingKitallowsyoutouseupto96uniqueindexesperlaneontherapidflowcell.ThisdoublestheindexingcapABIlityachievedwiththeon-boardclustergenerationprocessalone.Using2HiSeqRapidDuocBotSampleLoadingKitsfor2rapidflowcellsenablesupto384uniqueindexesperrunonaHiSeq2500inrapidrunmode.
Formanyapplications,it"snecessarytokeepsamplesphysicallydistinctratherthansimplyindexingtodistinguishonesamplefromanother.Fortheseapplications,theHiSeqRapidDuocBotSampleLoadingKitoffersanidealsolution.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn