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| CatalogueNumber | CYT-433 | |||||||||||||||
| Synonyms | Acrp30,AdipoQ,GBP-28,APM-1,ACDC. | |||||||||||||||
| Introduction | Adiponectin,alsoreferredtoasAcrp30,AdipoQandGBP-28,isarecentlydiscovered244aminoacidprotein,theproductoftheapM1gene,whichisphysiologicallyactiveandspecificallyandhighlyexpressedinadiposecells(Adipokine).Theproteinbelongstothesolubledefensecollagensuperfamily;ithasacollagen-likedomainstructurallyhomologouswithcollagenVIIIandXandcomplementfactorC1q-likeglobulardomain.APM-1formshomotrimers,whicharethebuildingblocksforhigherordercomplexesfoundcirculatinginserum. | |||||||||||||||
| Description | TheAcrp30HumaniscreatedasarecombinantproteinwithN-terminalfusionofHisTag.TheAdiponectinHis-TaggedFusionProtein,producedinE.coli,is26.4kDaproteincontaining230aminoacidresiduesoftheAcrp30Humanand12additionalaminoacidresidues-HisTag(underlined). | |||||||||||||||
| Source | EscherichiaColi. | |||||||||||||||
| PhysicalAppearance | FilteredWhitelyophilized(freeze-dried)powder. | |||||||||||||||
| Formulation | Acrp30Humanwasfiltered(0.4µm)andlyophilizedfrom0.5mg/mlin0.02MTrisbufferpH7.5,0.15MNaCl. | |||||||||||||||
| Solubility | Itisrecommendedtoadddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitoncellculture. | |||||||||||||||
| StABIlity | StorelyophilizedAcrp30Humanat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.ReconstitutedAcrp30Humancanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C. | |||||||||||||||
| Aminoacidsequence | MRGSHHHHHHGSGHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN. | |||||||||||||||
| Purity | Acrp30Humanpurityisgreaterthan95%asdeterminedbySDS-PAGE. | |||||||||||||||
| Usage | ProSpec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals. | |||||||||||||||
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