Cataloguenumber
ENZ-906
Synonyms
UBCH9,UbcM2,UBE2E3,Ubiquitin-ConjugatingEnzymeE2E3,UBCH9,Ubiquitin-ConjugatingEnzymeE2E3(HomologousToYeastUBC4/5),Ubiquitin-ConjugatingEnzymeE2E3(UBC4/5Homolog,Yeast),Ubiquitin-ConjugatingEnzymeE2-23KDa,UbiquitinCarrierProteinE3,Ubiquitin-ProteinLigaseE3,EC6.3.2.19,Ubiquitin-ConjugatingEnzymeE2E3,UBCE4.
Introduction
UBE2E3orUbiquitin-conjugatingenzymeE2E3ispartoftheE2ubiquitinconjugatingenzymefamily.UBE2E3isneededforthedestructionofmitoticcyclinsandforcellcycleprogression.Theubiquitinationprocesscovalentlyattachestoashortproteinof76aminoacidscalledubiquitin,toalysineresidueonthetargetprotein.Whenaproteinhasbeentaggedwithoneubiquitinmolecule,otherroundsofubiquitinationformapolyubiquitinchainthatisrecognizedbytheproteasome"s19Sregulatoryparticle,triggeringtheATP-dependentunfoldingofthetargetproteinwhichgrantspassageintotheproteasome"s20Scoreparticle,whereproteasesdegradethetargetintoshortpeptidefragmentsforrecyclingbythecell.
Description
UBE2E3HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining230aminoacids(1-207a.a)andhavingamolecularmassof25.3kDa.
UBE2E3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
UBE2E3proteinsolution(1mg/ml)containingPhosphatebufferedsaline(pH7.4),20%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan80.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
