产品资料

Prospec/Carbonic Anhydrase 2 Human/ENZ-420/1mg

Cataloguenumber

ENZ-420

Synonyms

Carbonicanhydrase2,Carbonatedehydratase2,CarbonicAnhydraseII,CA-II,CarbonicanhydraseC,CAC,CA2,CAII,Car2.

Introduction

TheenzymeCarbonicanhydraseIIhavinganaccessionnumberofNP_414668isalsocalledcarbonatedehydratasewhichispartoftheenzymefamilythatcatalysesrapidinter-conversionofcarbondioxide&watertobicarbonate,carbonicacidandprotons(CO2+H2O?HCO3?+H+),areactionthatoccursratherslowlyintheabsenceofacatalyst.Themajorityofcarbonicanhydrasesencloseazincionintheiractivesiteandthereforeisclassifiedasmetalloenzymes.
ThemostimportantfunctionofCarbonicanhydraseisknowntopreserveacid-basebalanceinbloodandothertissues,andtohelptransportcarbondioxideoftissues.Carbonicanhydraseshavebeenfoundinallkingdomsoflife.Carbonicanhydrasehas3differentclasses:alpha,betaandgammawhichshareverylittlesequenceorstructuralsimilarity,thusfartheyallperformthesamefunctionandrequireazincionattheactivesite.Mammaliancarbonicanhydraseismonomericandbelongstothealphaclass.Plantcarbonicanhydraseisdimericandbelongstothebetaclass.
Methane-producingbacteriacarbonicanhydraseistrimericandgrowsinhotspringswhichformsthegammaclass.

Description

Carbonicanhydrase2HumanRecombinantproteinproducedinE.Colicontaining260aminoacids(1-260)andhavingamolecularmassof29.2kDa.
TheCarbonicanhydrase2ispurifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredcolorlesssolution.

Formulation

CarbonicAnhydrase2proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,50mMNaCland10%glycerol.

StABIlity

Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.

Aminoacidsequence

MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

BIOLOGicalActivity

Specificactivityis50-70nmoles/min/µgandwasobtainedbymeasuringtheincreaseintheamountofp-nitrophenolbyitsesteraseactivity.Specificactivityisdefinedastheamountof

p-nitrophenolthat1ugofenzymecanreduceat25Cfor1minute.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒