QED Bioscience/Recombinant Human HspA9 (Hsp70 protein 9)/11118P1000/1 Ea

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Formulation:Sterile-filteredcolorlesssolutionin20mMTris-Hcl,pH8.0,0.5mMDTTand10%glycerol.Purifiedbyproprietarychromatographictechniques.

BACKGROUND

HspA9,amemeroftheHsp70family,encodesaheat-shockcognateproteinthatisinvolvedinthecontrolofcellproliferationandactsasachaperone.HspA9islocatedonchromosome5,bandq31,aregionthatisoftendeletedinmyeloidleukemiasandmyelodysplasia,makingitacandidatetumorsuppressorgene.HspA9suppressesnucleartranslocation,transcriptionalactivation,andcontrolcentrosome-duplicationfunctionsofp53.

RecombinanthumanHspA9producedinE.coliisasingle,non-glycosylatedpolypeptidecontaining654aminoacids(aa47-679)withamolecularweightof71kDa.Itisexpressedwitha20-aminoacidHis-tagattheN-terminus.

Source:EscherichiacoliPurity:Greaterthan95%asdeterminedbySDS-PAGE.Accessionnumber:P38646Aminoacidsequence:

GSSHHHHHHSSGLVPRGSHMASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ.

Storeat4oCifentirevialwillbeusedwithin2-4weeks.Storeatorbelow-20oCforlongerperiodsoftime.Additionofacarrierprotein(suchas0.1%HSAorBSAisrecommendedforlong-termstorage.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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