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QED Bioscience/Acinus (NP)/2241/1 Ea


Anti-Acinus(NP)Antibody


ORDERINGINFORMATION

CatalogNo.:2241

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.


BACKGROUND

AnewinducerofchromatincondensationwasrecentlyidentifiedanddesignatedAcinus(forApoptoticChromatinCondensationInducerintheNucleus).

Acinusiscleavedbycaspase-3andanadditionalproteasetogenerateasmallactivepeptide,p17,whichcauseschromatincondensationinvitrowhenitisaddedtopurfiednuclei.Acinusalsoinducesapoptoticchromatincondensationincells.

Acinusisubiquitouslyexpressed.ThreedifferentsplicedformsofAcinushavebeenidentifiedinhumanandmouseandaredesignatedAcinusL,AcinusS,andAcinusS’.


SPECIFICATIONSUMMARY

Antigen:Peptidecorrespondingtoaa994-1009ofhumanAcinusL,aa236-251ofhumanAcinusSandaa267-282ofhumanAcinusS’whichareidenticaltothoseofmouseAcinus.ThesesequencesarelocatedneartheN-terminusofactivepeptidep17.

HostSpecies:Rabbit

Preservatives:0.02%sodiumazide.

SPECIFICITY

ThisantibodyrecognizeshumanandmouseAcinus(220kD).


APPLICATIONS

Immunoblotting:useat1-10ug/ml

WesternblotanalysisofAcinusinK562wholecelllysatewithAcinusantibody(NP)at1μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:K562wholecelllysate.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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