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QED Bioscience/Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rHuGMCSF)/45104P


RecombinantHumanGranulocyteMacrophage-ColonyStimulatingFactor(rHuGM-CSF)


CatalogNumber

Size

45104P-5

5ug

45104P-20

20ug

45104P-1000

1000ug

CatalogNumber

Size

45104P-5

5ug

45104P-20

20ug

45104P-1000

1000ug

ORDERINGINFORMATION


Formulation:Sterilefilteredandlyophilizedafterdialysisagainst2mMsodiumphosphate,pH7.4.Purifiedbyproprietarychromatographictechniques.


BACKGROUND

GM-CSFwasfirstidentifiedasagrowthfactorthatsupportsinvitrocolonyformationofgranulocyte-macrophageprogenitorcells.ItisproducedinresponsetoimmuneorinflammatorystimulibyactivatedTcells,Bcells,macrophages,mastcells,fibroblasts,andalveolarepithelialcells.GM-CSFisa22kDaprotein,144aminoacids,composedoffourbundlealphahelices.


DESCRIPTION

RecombinanthumanGM-CSFproducedinE.coliisasingle,non-glycosylatedpolypeptidecontaining127aminoacidswithamolecularweightof14,477Da.

Accessionno.:P04141.1Purity:>98%asdeterminedbyRP-HPLCandSDS-PAGE.AMINOACIDSEQUENCE

ThefirstfiveN-terminalaminoacidsareAla-Pro-Ala-Arg-Ser.N-terminalmethionineisremovedenzymatically.


BIOLOGICALACTIVITY

ED50bydose-dependentstimulationofproliferationofhumanTF-1cells(humanerythroleukemicindicatorcellline)is<0.1ng/mlwhichcorrespondstoaSpecificActivityof11,100,000IU/mg.


STORAGEANDSTABILITY

ReconstitutelyophilizedGM-CSFinsteriledistilledH2Oatnotlessthan100ug/ml.Thiscanbefurtherdilutedinotheraqueoussolutions.

Storelyophilizedproductdesiccatedbelow-18oC.Afterreconstitution,GM-CSFmaybestoredat4oCfor2-7daysandbelow-18oCforfutureuse.Forlongtermstorage,additionof0.1%humanserumalbuminorbovineserumalbuminisrecommended.Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.


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