QED Bioscience/Recombinant Human InsulinLike Growth Factor I (rHuIGFI)/20802P20/1 Ea

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Format:Sterilefiltered,lyophilized.

Purification:Chromatographic

RecombinantHumanIGF-1producedin

E.coliisasingle,non-glycosylatedpolypeptidechaincontaining70aminoacidswithamolecularmassof7,655daltons.Thisproducthasfullbiologicalactivity.

Source:EscherichiacoliStabilizers:NonePreservatives:None

Purity:Greaterthan98%byRP-HPLC,anion-exchangechromatography;reducingandnon-reducingSDS-PAGE,silverstain.

Dimers/Aggregates:<1%bySDS-PAGE

BiologicalActivity:Fullybiologicallyactivecomparedtoreferencestandards.Specificactivityis1x106IU/mg.

Biologicalactivityat0.2-20ng/ml.

Endotoxin:<0.1ng/ug

ProteinContent:Determinedspectrophotometricallyat280nm(0.502ext.coeff),andbyRP-HPLCanalysisagainstacalibratedstandard.

Reconstitutethelyophilizedproductinsterilewaternotlessthan100ug/ml.Productwasdialyzedagainst50mMacetatepriortolyophilization.

Lyophilizedproductisstableatroomtemperaturefor3weeks.Storelyophilizedproductdesiccatedat-20°C.Uponreconstitution,storeat4°Cfor2-7daysoraliquotandstoreat-20°C.Forlong-termstorageaddeitherHSAorBSAto0.1%.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

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