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Hsp90andthe94kDaglucose-regulatedprotein,Grp94,aremajormolecularchapeonesofthecytosolandendoplasmicreticulum.Inmammaliancells,thereareatleasttwoHsp90isoforms,Hsp90αandHsp90β,whichareencodedbyseparategenes.AllknownmembersoftheHsp90familyarehighlyconserved,especiallyintheN-terminalandC-terminalregions.Intheabsenceofstress,Hsp90isanessentialcomponentofcellularprocessessuchashormonesignalingandcellcyclecontrol.
Severalregulatoryproteinssuchassteroidreceptors,cellcyclekinasesandp53havebeenidentifiedassubstratesofHsp90.
Thisantibodyrecognizeshuman,mouse,rat,rabbit,bovine,porcine,andchickenHsp90α(90kDa).
Immunoblotting:useat1-5ug/ml.Abandof
~90kDaisdetected.Immunoprecipitation:5ugon20ulProteinA-Sepharose+100ulsample.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Heat-shockedHeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
SchuhSetal.1985JBiolChem260:14292-14296;SullivanWPetal.1985
Biochemistry24:4214-4222.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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