QED Bioscience/Akt (PhosphoSer473)/43054/1 Ea

ORDERINGINFORMATION

CatalogNo.:43054

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Akt,alsoknownasAkt1andProteinkinaseB(PKB),isaserine/threonine-specificproteinkinasethatplaysakeyroleinmultiplecellularprocessessuchasglucosemetabolism,apoptosis,cellproliferation,transcriptionandcellmigration.Akt1isalsoabletoinduceproteinsynthesispathwaysandis,therefore,akeysignalingproteininthecellularpathwaysthatleadtoskeletalmusclehypertrophy,andgeneraltissuegrowth.Sinceitcanblockapoptosis,andtherebypromotecellsurvival,Akt1hasbeenimplicatedasamajorfactorinmanytypesofcancer.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsitesofserine473(Q-F-S(p)-Y-S)derivedfromhumanAktandconjugatedtoKLH.

Accessionno.:P31749,NP_001014431.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratAktonlywhenphosphorylatedatserine473.

APPLICATION

Immunoblotting:useatdilutionof1:500-1:1,000.Abandof

~60kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofAkt(phospho-Ser473)inextractsof293cellsuntreatedortreatedwithEGForcalfintestinalphosphatase.

DetectionofAkt(phospho-Ser473)inparaffin-embeddedhumanbreastcarcinomatissue.

DetectionofAkt(phospho-Ser473)inparaffin-embeddedhumanlungcarcinomatissue.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.