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DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
SPECIFICATIONSUMMARYSTORAGEANDSTABILITYAntigen:50101,50102,50103:IM-9Theseantibodiesarestableforatleastlymphocytes.50104:humanplacentalone(1)yearat-20oCto-70oC.
insulinreceptor.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles
50102,50103=IgG1;50104=IgG2bSoosetal.(1986)BiochemJ235:199;
Allfourclonesrecognizethehumaninsulinreceptor;low-levelofcross-reactivitywithbovineandrabbitinsulinreceptor.
Conc.forhalf-maximaleffect(nM)
SubunitCat.No.Specificity | %InhibitionofInsulinBindingIM-9Adipocytes | BiologicalInhibitionof ActivityInsulinBinding | StimulationofLipogenesis |
50101(83-14)α | 8090 | Insulin-like0.05 | 0.01 |
50102(83-7)? | 010 | Insulin-like- | 1.0 |
50103(47-9)α | 9298 | Inhibitsinsulin0.2 | - |
50104(18-44)β | 3540 | Insulin-like0.2 | 0.3 |
Cat.No. 50101(83-14) | Immunoprecipitation +++ | WesternBlot - | ReceptorKinaseAssay Activatesreceptorkinase |
50102(83-7) | + | - | Activatesreceptorkinase |
50103(47-9) | - | - | Blocksactivationbyinsulin |
50104(18-44) | + | + | Activatesreceptorkinase |
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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