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QED Bioscience/Ras/11068200/1 Ea


Anti-RasAntibody


CatalogNumber

Quantity

11068-50

50ug

11068-200

200ug

CatalogNumber

Quantity

11068-50

50ug

11068-200

200ug

ORDERINGINFORMATION


BACKGROUND

MonomericG-proteinRasfunctionsasamolecularswitchlinkingreceptorandnonreceptortyrosinekinaseactivationtoanumberofdownstreamsignalingpathways.Oncogenicmutations,whichoccurinapproximately30%ofallcasesofhumancancer,leadtothestimulationofproliferationandtheinhibitionofapoptosis.


SPECIFICATIONSUMMARY

Antigen:A13-residuesyntheticpeptidefromtheeffectorbindingloopofH-,K-,andN-Ras,residues31-43(EYDPTIEDSYRKQ((C).

HostSpecies:Rabbit

Format:AffinityPurified

Buffer:PBS,pH7.2,50%glycerol,0.09%sodiumazide.


SPECIFICITY

Thisantibodyrecognizeshuman,mouse,ratandbovineRas.Itdetectsabandof21-23kDabyWesternblot.

APPLICATIONS

Immunoblotting:useat1–4ug/ml.

WesternblotanalysisofRas:Lane1:MWMarker,Lane2:HeLalysate,MouseBrainLysate,RatBrainLysate.

Positivecontrol:HeLacelllysate.


Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.


DILUTIONINSTRUCTIONS

DiluteinPBSormediawhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.


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