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ThetetrapeptideKDEL,locatedatthecarboxy-terminalsequencesofluminalproteins,isaretrievalmotifessentialfortheprecisesortingoftheseproteinsalongthesecretorypathway.KDELproteinsperformessentialfunctionsintheendoplasmicreticulum(ER)relatedtoproteinfoldingaswellasassembly.ThelocalizationofchaperonesandothersolubleproteinstotheERisachievedbytheircontinuousretrievalfrompost-ERcompartmentsbytheKDELreceptorwhichisamembraneproteinlocalizedintheGolgiapparatus.
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
Stableforatleastoneyearatorbelow
-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:Useat4ug/ml.ReactivitywithproteinscorrespondingtotheapparentmolecularmassofGrp78andGrp94insamplesfrommammals,birds,insects,andplants.
Lane1:Grp78(BiP)recombinantprotein,Lane2:RK-13,Lane3:Mouselivermicrosomes,Lane4:Ratlivermicrosomes,Lane5:Heat-shockedHeLacelllysate.
Immunohistochemistry:Useat5-10ug/ml.
Humanspleenstainedwith#11076-200at10ug/ml.
Immunofluorescence:
StainingofERinmouseC2C12myoblaststransfectedwithwild-typemouseADAM12with
#11076-200at10ug/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.