QED Bioscience/CIDEB (mouse CT)/2091/1 Ea

CatalogNo.:2091

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

DFF45-relatedproteinsCIDE-AandCIDE-Bhaverecentlybeenidentified.CIDEcontainsanewtypeofdomaintermed

CIDE-N,whichhashighhomologywiththeregulatorydomainsofDFF45/ICADandDFF40/CAD.ExpressionofCIDE-Binducesapoptosis,whichisinhibitedbyDFF45.

CIDE-BisaDFF45-inhibitableeffectorthatpromotescelldeathandDNAfragmentation.CIDE-Bisexpressedmainlyinliverandatlowerlevelsinspleen,kidney,peripheralbloodlymphocytes,andbonemarrow.

Antigen:Peptidecorrespondingtoaa204-219ofmouseCIDE-B(accessionno.

AF041377).

HostSpecies:Rabbit

Stabilizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizesmouseCIDE-B(25kDa)anddoesnotcross-reactwithCIDE-A.

Immunoblotting:useat2ug/ml.

WesternblotanalysisofCIDE-BinmurinelivertissuelysatewithCIDE-Bantibodyat2ug/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationfortheirapplication.

Positivecontrol:Tissuelysateofmouseliver.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.