QED Bioscience/Caspase8/11026/1 Ea

CatalogNo.:11026

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Caspasesareafamilyofcysteineproteases.Caspase-8isaninitiatorcaspasethatwasidentifiedasamemberoftheFas/APO-1death-inducingsignalingcomplex.TheadaptormoleculeFADDcouplesprocaspase-8totheFasreceptordeathdomain;subsequentoligomerization

promotesprocaspase-8autoactivation.FLIP,acatalyticallyinactivecaspase-8-likemoleculeinhibitstheseinteractionsandthuscaninhibitapoptosis.

Antigen:Syntheticpeptidecorrespondingto16aminoacidsattheC-terminusofhumanCaspase-8isoformA(accessionno.NP_001219).

HostSpecies:Rabbit

Stabilizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizeshuman,mouse,andratCaspase-8(18kDa).

Immunoblotting:useat0.5-2ug/ml.

WesternblotanalysisofCaspase-8inJurkatcelllysatewithCaspase-8antibodyat(A)0.5,(B)1,and(C)2μg/ml.

Positivecontrol:Jurkatcelllysate.

Immunocytochemistry:useat2ug/ml.

ImmunocytochemicalstainingofCaspase-8inJurkatcellswithCaspase-8antibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.