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| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 150 µl |
| Equipment | Microcentrifuge and centrifuge or vacuum source. |
| Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 100 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
We recommend no more than 5 times as binding efficiency might decrease.
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor"s kits.”
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
“It was a very simple procedure and it gave a concentration ten times the original amount.”
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
| D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
| D3004-4-10 | DNA Elution Buffer | 10 ml | $14.00 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $90.00 | |
| C1001-500 | Collection Tubes | 500 Pack | $52.00 | |
| C1016-25 | Zymo-Spin V Columns w/ Reservoir | 25 Pack | $67.00 | |
| C1016-50 | Zymo-Spin V Columns w/ Reservoir | 50 Pack | $105.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 |
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vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
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