ODN 2088 | Unit size | Cat. code | Docs | Price |
---|---|---|---|---|
TLR9 antagonist - Mouse preferred | 200 µg 1 mg 5 mg | tlrl-2088 | TDSMSDS | Please contact our distributor Add to favorite |
Toll-like receptor 9 (TLR9) recognizes specific unmethylated CpG oligonucleotide (ODN) sequences that distinguish microbial DNA from mammalian DNA.
Studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs [1].
These sequences are characterized by three consecutive Gs downstream of a C or A, addition of a fourth G (G-tetrads) increases the inhibitory capability.
The most potent inhibitory sequences are (TTAGGG)4 found in mammalian telomeres [2] and ODN 2088 which derives from a stimulatory ODN by replacement of 3 bases [3].
Inhibitory ODNs act by disrupting the colocalization of CpG ODNs with TLR9 in endosomal vesicles without affecting cellular binding and uptake.
References:
1. Krieg AM. et al., 1998. Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs. Proc Natl Acad Sci USA. 95(21): 12631-6.2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.3. Stunz LL. et al., 2002. Inhibitory oligodinucleotides specifically block effects of stimulatory CpG oligonucleotides in B cells. Eur J Immunol. 32(5): 1212-22.
Back to the topSpecificity: murine TLR9 antagonist
Working concentration: 100 nM - 10 µM
Solubility: 2.5 mg/ml in water
ODN 2088 sequence5’- tcc tgg cgg gga agt -3’ (15 mer)Note: Bases are phosphorothioate (nuclease resistant).
Quality control:
ODN 2088 is provided lyophilized and is available in three quantities:
ODN 2088 is shipped at room temperature.
Upon receipt, store at -20 °C.
Back to the top2018 Diabetologia. DOI: 10.1007/s00125-018-4705-0
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