ODN TTAGGG (A151) | Unit size | Cat. code | Docs | Price |
---|---|---|---|---|
TLR9 antagonist - Human preferred | 200 µg 1 mg | tlrl-ttag151 | TDSMSDSDATA | Please contact our distributor Add to favorite |
ODN TTAGGG (A151), also known simply as A151, is a synthetic oligonucleotide (ODN) containing 4 repeats of the immunosuppressive TTAGGG motif commonly found in mammalian telomeric DNA [1].
This ODN was initially identified as a TLR9 antagonist that inhibits immune activation by CpG-containing ODNs [2].
Subsequently, additional targets for this inhibitor were found: by binding to the cytosolic DNA sensors (CDSs) AIM2 and IFI16, A151 prevents AIM2 inflammasome activation [3,4].
This ODN is also described as a cGAS inhibitor, acting through competition with DNA [1].
Overall, these findings show that ODN TTAGGG (A151) is a multiple pattern recognition receptors (PRR) suppressor that can prove useful for immunomodulatory studies.
References:
1. Steinhagen F. et al., 2017. Suppressive oligodeoxynucleotides containing TTAGGG motifs inhibit cGAS activation in human monocytes. Eur J Immunol. DOI: 10.1002/ eji.201747338.2. Gursel I. et al, 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.3. Eichholz K. et al., 2016. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 12(9):e1005871.4. Kaminski J. et al., 2013. Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J Immunol. 191(7):3876-83.
Working concentration: 100 nM - 10 µM
Solubility: 5 mg/ml in water
Molecular weight: 7944 g/mol
ODN TTAGGG sequence5’- tt agg gtt agg gtt agg gtt agg g -3’ (24 mer)Note: Bases are phosphorothioate-linked (nuclease resistant).
Quality control:- Biological activity has been tested using HEK-Blue™ TLR9 cells.- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Back to the topODN TTAGGG (A151) is provided lyophilized and is available in two quantities.
tlrl-ttag151:
tlrl-ttag151-1:
ODN TTAGGG (A151) is shipped at room temperature.
Upon receipt, store at -20 °C.
Back to the top2020 J Invest Dermatol. DOI: 10.1016/j.jid.2019.09.029
Kopfnagel V. et al.
2017 Nat Commun. 8(1):1282.
Rivera-Correa J. et al.
2015 J Exp Med. 212(2):129-37.
Flierl U, Nero TL, Lim B, Arthur JF, Yao Y, Jung SM, Gitz E, Pollitt AY, Zaldivia MT, Jandrot-Perrus M, Schäfer A, Nieswandt B, Andrews RK, Parker MW, Gardiner EE, Peter K.
2014 J Leukoc Biol.95(6):983-91.
Deknuydt F, Nordström T, Riesbeck K.
2013 Ann Rheum Dis. 72(3):418-26.
Chamberlain ND, Kim SJ, Vila OM, Volin MV, Volkov S, Pope RM, Arami S, Mandelin AM 2nd, Shahrara S.
2012 Blood 119(12):2810-2818
Kim YC, Bhairavabhotla R, Yoon J, Golding A, Thornton AM, Tran DQ, Shevach EM
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