Type
Recombinant
Description
EGFisapotentgrowthfactorthatstimulatestheproliferationofvariousepidermalandepithelialcells.Additionally,EGFhasbeenshowntoinhibitgastricsecretion,andtobeinvolvedinwoundhealing.EGFsignalsthroughareceptorknownasc-erbB,whichisaclassI tyrosinekinasereceptor.ThisreceptoralsobindswithTGF-αandVGF(vacciniavirusgrowthfactor).RecombinantMurineEGFisa6.0 kDaglobularproteincontaining53 aminoacidresidues,including3 intramoleculardisulfide bonds.
AminoAcidSequence
NSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR
Source
E.coli
Purity
98%
BiologicalActivity
TheED50wasdeterminedbyacellproliferationassayusingBALB/c3T3cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C