Type
Recombinant
Description
Total193 AA.MW:22 kDa(calculated).UniProtKBacc.no.P23284(Asp34-Glu216).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
MKHHHHHHASDEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis:> 95%
SDS-PAGEGel
14 %SDS-PAGEseparationofHumanCyclophilinB:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5 μg/lane
3.non-reducedandnon-boiledsample,2.5 μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLsolutionin20 mMTrisbuffer,50 mMNaCl,pH 7.5.
Reconstitution
Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.