Description
GHHumanRecombinantproducedinE.coliisasingle,non-glycosylated,polypeptidechaincontaining192 aminoacidsandhavingamolecularmassof22260.01 Dalton.GrowthHormoneispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Phe-Pro-Thr-Ile.
Source
E.coli
Purity
Greaterthan98.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
Biovendor’s GHiscontains3 units/mg.
Formulation
TheGH(1 mg/ml)waslyophilizedafterextensivedialysesagainst8 mgmannitoland0.34 mgsodiumphosphatebuffer(0.02 mgsodiumphosphatemonobasic&0.32 mgsodiumphosphatedibasic).
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedGHalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionHGHshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.