Type
Recombinant
Description
PlateletActivatingFactor(PAF)isaBIOLOGicallyactivephospholipid,whichexertsprimarilyproinflammatoryactivitiesbyspecificallysignalingthroughG-protein-coupledreceptorsonplatelets,neutrophils,andmonocytes.PlateletActivatingFactorAcetylhydrolase(PAF-AH)isasecretedproteinthatmediatesPAFactivitybyspecificallycatalyzinghydrolysisofthe“sn2”esterbond,resultingintheconversionofPAFtothebiologicallyinactivelyso-PAF.PAF-AHcanalsointeractwithLDLparticlestoinducethehydrolysisofLDL-associated,oxidizedphospholipids,generatinglysophosphatidylcholine(lyso-PC)andotherlysophospholipids.RecombinantHumanPAF-AHisa420 aminoacidglycoproteinwhichmigrateswithanapparentmolecularmassof47–55kDabySDS-PAGEanalysis.RecombinanHumanPAF-AHhasacalculated,theoreticalmeolecularweightof47.8 kDa.
AminoAcidSequence
FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNVAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYN
Source
HEK293cells
Purity
95%
BiologicalActivity
MeasuredbyitsABIlitytocleaveaPAFanaloginachromogenicsubstratelinkedassay.AtaPAF-AHconcentrationof10.0 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately2 minutes.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C