Type
Recombinant
Description
LAG-1 isCCchemokinethatsignalsthroughtheCCR5receptor.LAG-1 isidenticaltoMIP-1β(ACTIIisotype)exceptfortwoaminoacidsubstitutions;arginineforhistidineatposition22 andserineforglycineatposition47 ofthematureprotein.LAG-1 chemoattractsmonocytes,andexhibitsactivityasanHIV-suppressivefactor.RecombinantHumanLAG-1 isa7.7 kDaproteincontaining69 aminoacidresidues.
AminoAcidSequence
APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN
Source
E.coli
Purity
98%
BiologicalActivity
Determinedbyits abilitytochemoattractCCR5transfectedcellsusingaconcentrationof0.1–10.0 ng/ml
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C