Type
Recombinant
Description
Total141 AA.MW:16.2 kDa(calculated).UniProtKBacc.no.Q96NZ9(Val21–Gln151).N-TerminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
MKHHHHHHASVPAPKVPIKMQVKHWPSEQDPEKAWGARVVEPPEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGHVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis: >85%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanPRAP1:
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/ lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedin0.5 mg/mlin0.05 Mphosphatebuffer,0.075 MNaCl, pH 7.4
Reconstitution
Add200μlofdeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.