Type
Recombinant
Description
NogginbelongstoagroupofdiffusibleproteinsthatbindtoligandsoftheTGF-βfamily,andregulatetheiractivitybyinhibitingtheiraccesstosignalingreceptors.NogginwasoriginallyidentifiedasaBMP-4 antagonistwhoseactionwascriticalforproperformationoftheheadandotherdorsalstructures.Consequently,nogginhasbeenshowntomodulatetheactivitiesofotherBMPsincludingBMP-2,–7,–13,and –14.Targeteddeletionofnoggininmiceresultsinprenataldeath,andarecessivephenotypedisplayingaseverelymalformedskeletalsystem.Conversely,transgenicmiceover-expressingnoggininmatureosteoblastsdisplayimpairedosteoblasticdifferentiation,reducedboneformation,andsevereosteoporosis.RecombinantMurineNogginisa46.4 kDadisulfide-linkedhomodimerconsistingoftwo206 aminoacidpolypeptidechains.
AminoAcidSequence
MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC
Source
E.coli
Purity
95%
BiologicalActivity
Determinedbyitsabilitytoinhibit5.0 ng/mlofBMP-4 inducedalkalinephosphataseproductionbyATDC5chondrogeniccells.TheexpectedED50forthiseffectis1.0–2.0 ng/mlofNoggin.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Note:DuetosolubilityreasonstheproteinshouldbekeptatlowpH.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C