Type
Recombinant
Description
IL-15 isanimmunomodulatingcytokinethatstimulatestheproliferationofTlymphocytesandsharesmanybiologicalpropertieswithIL-2. IL-15 exertsitsbiologicalactivitiesprimarilyonTcells.Itisalsoessentialinthedevelopment,survivalandactivationofNKcells.IncreasedexpressionofIL-15 hasbeenlinkedtowithrheumatoidarthritis,inflammatoryboweldisease,anddiseasesaffiliatedwithretrovirusesHIVandHTLV-I.HumanIL-15 isbiologicallyactiveonmousecellsasmeasuredbythedose-dependentstimulationoftheproliferationofmouseCTLLcells.RecombinantHumanIL-15 isa12.9 kDaproteinconsistingof115 aminoacidresidues.
AminoAcidSequence
MNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50 asdeterminedbythedose-dependentstimulationoftheproliferationofCTLL-2 cellswasfoundtobe≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C