Type
Recombinant
Description
LIGHTbelongstotheTNFfamilyofligands,andcansignalthroughtheherpesvirusentrymediatortypeA receptor(HVEM,TNFRSF14),LTβR,orbindtoadecoyreceptor,DcR3.Itisexpressedinsplenocytes,activatedPBL,CD8+tumorinfiltratinglymphocytes,granulocytes,andmonocytes.LIGHThastheabilitytoactiveNF-κB,toco-stimulatetheactivationoflymphocytesandtoinduceapoptosisincertainhumantumorcells.ThehumanLIGHTgeneencodesfora240 aminoacidtypeIItransmembraneproteincontaininga37 aminoacidcytoplasmicdomain,a21 aminoacidtransmembranedomain,anda182 aminoacidextracellulardomain.RecombinantHumanLIGHThasacalculatedmassof19.3 kDa,containing177 aminoacidresidues.Duetoglycosylation,LIGHTmigratesbetween20.0–22.5 kDabySDS-PAGEundernon-reducingconditions.
AminoAcidSequence
RLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV
Source
Hi-5Insectcells
Purity
95%
BiologicalActivity
DeterminedbyitscytotoxiceffectonHT-29 cells(humancolonadenocarcinomacells)inthepresenceofhuman IFN-ɣ.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C