Type
Recombinant
Description
CXCL6,alsoknownasGCP-2 inhumans,isaconnectivetissue-derivedCXCchemokinethatcansignalthroughtheCXCR1andCXCR2receptors.HumanGCP-2,whichiscross-reactivewithmurinecells,selectivelyattractsneutrophils,andhasalsobeenshowntoexertanti-angiogenicactivity.RecombinantHumanGCP-2 isa7.9 kDaproteincontaining72 aminoacidresidues.
AminoAcidSequence
VLTELRCTCLRVTLRVNPKTIGKLQVFPAGPQCSKVEVVASLKNGKQVCLDPEAPFLKKVIQKILDSGNKKN
Source
E.coli
Purity
98%
BiologicalActivity
Determinedbyitsabilitytochemoattracthumanneutrophilsusingaconcentrationrangeof10.0–50.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C