Type
Recombinant
Description
Total283 AA.MW:30.5 kDa(calculated).UniProtKBacc.No.Q96D42(Ser21–Thr288).N-terminallinker(2 extraAA),C-terminallinker(2 extraAA)andC-terminalHis-tag(6 extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
ASSVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPMTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTANTTKLHHHHHH
Source
HEK293
Purity
Purityasdeterminedbydensitometricimageanalysis:>90 %
SDS-PAGEGel
12 %SDS-PAGEseparationofHumanKIM-1(HEK):
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane
Endotoxin
<1.0 EU/μg
Formulation
Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mlsolutioninphosphatebufferedsaline,5 %w/v trehalose.
Reconstitution
Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Attemperature2–8°C.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.