Type
Recombinant
Description
Total122 AA.MW:13.6 kDa(calculated).UniProtKBacc.no.P61366.N-TerminalHis-tag16 AA(highlighted).
AminoAcidSequence
MRGSHHHHHHGMASHMVDVTTTEAFDSGVIDVQSTPTVREEKSATDLTAKLLLLDELVSLENDVIETKKKRSFSGFGSPLDRLSAGSVDHKGKQRKVVDHPKRRFGIPMDRIGRNRLSNSRG
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis: >95%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanOsteocrin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane
Endotoxin
<1.0 EU/μg
Formulation
Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.05 MAcetatebuffer, pH=4.0
Reconstitution
Add0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.