Type
Native
Description
Nativeproteinisolatedfromhumanurine,590 AA,64,265 kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
DTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHCHALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAHRTLDEYWRSTEYGEGYACDTDLRGWYRFVGQGGARMAETCVPVLRCNTAAPMWLNGTHPSSDEGIVSRKACAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEECSIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFTVRMALFQTPSYTQPYQGSSVTLSTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPHTRDSTIQVVENGESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSRVLNLGPITRKGVQATVS
Source
Humanurine
Purity
>95%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanUromodulin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/ lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLindeionized water.
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
GFCtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
AlldonorsofurinesamplesusedforproteinpreparationweretestedandfoundnegativeforHBsAg,anti-HCV,HIVAg/Ab,andsyphilis.Sincenotestcanabsolutelyassuretheabsenceofallinfectiousagents,thisproductshouldbehandledasapotentialbiohazard.Thisproductisintendedforresearchuse only.