Type
Recombinant
Description
IL-1aisanon-secreted,proinflammatorycytokineproducedinavarietyofcells,includingmonocytes,tissuemacrophages,keratinocytes,andotherepithelialcells.BothIL-1aandIL-1bbindtothesamereceptorandhavesimilar,ifnotidentical,biologicalproperties.ThesecytokineshaveabroadrangeofactivitiesincludingthestimulationofthymocyteproliferationbyinducingIL-2 release,B-cellmaturationandproliferation,mitogenicFGF-likeactivity,andthereleaseofprostaglandinandCollagenasefromsynovialcells.However,whereasIL-1bisasecretedcytokine,IL-1aispredominantlyacell-associatedcytokine.RecombinantRatIL-1aisa17.7 kDaproteincontaining155 aminoacidresidues.
AminoAcidSequence
MAPHSFQNNLRYKLIRIVKQEFIMNDSLNQNIYVDMDRIHLKAASLNDLQLEVKFDMYAYSSGGDSKYPVTLKVSNTQLFVSAQGEDKPVLLKEIPETPKLITGSETDLIFFWEKINSKNYFTSAAFPELLIATKEQSQVHLARGLPSMIDFQIS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentproliferationofmurineD10Scellsis0.005 ng/ml,correspondingtoaspecificactivityof2×108units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C