Type
Recombinant
Description
Matrixmetalloproteinases(MMPs)areafamilyofendoproteasesthatrequirezincandcalciumforexpressingcatalyticactivity.Theseenzymesplayacentralroleinthemaintenanceandremodelingoftheextracellularmatrix.Elevatedexpressionoftheiractivity,causedeitherbyup-regulationoftheirexpressionordown-regulationoftheircognateinhibitors,hasbeenimplicatedinvariousdegenerativedisorders,includingarthritis,cardiovasculardisease,skeletalgrowth-platedisorders,andcancermetastasis.MMP-2 isasecretedCollagenasewithspecificitytowardTypeIV,V,VII,andXcollagens.RecombinantHumanMMP-2 isa62.0 kDaproteincontainingtheentirecatalyticN-terminaldomainandtheC-terminaldomain(552 aminoacids).
AminoAcidSequence
MYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC
Source
E.coli
Purity
98%
BiologicalActivity
MMP-2 activitywasmeasuredbyitsabilitytocleaveachromogenicpeptideMMP-2 substrateatroomtemperature.AtanMMP-2 concentrationof2.5 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately25 minutes.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C