Type
Recombinant
Description
TGF-βfamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesisandapoptosis.Asimpliedbytheirname,BMPsinitiate,promote andregulatethedevelopment,growthandremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodelingandmaintenanceofavarietyofothertissuesandorgans.IncreasingevidenceindicatesthatBMP-SmadsignalinghasatumorsuppressingactivityandthatBMPscaninhibittumorgrowth.BMP-6 isabnormallyexpressedinbreastcancercelllines;however,itsfunctioninpromotingbreastcancerdevelopmentisunknown.RecombinanthumanBMP-6 isa26.2 kDahomodimericglycoproteinconsistingoftwo117 aminoacidsubunits,whichcorrespondtoaminoacidresidues397 to513 ofthefull-lengthBMP-6 precursor.
AminoAcidSequence
VSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPEYVPKPCCAPTKLNAISVLYFDDNSNVILKKYRNMVVRACGCH
Source
HEK293cells
Purity
95%
BiologicalActivity
DeterminedbyitsabilitytoinducealkalinephosphataseproductionbyATDC-5 cells.TheexpectedED50thiseffectis0.03–0.06 μg/ml
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C