Type
Recombinant
Description
ArginineDeiminase(ADI)isamicrobialenzymefromMycoplasmaproducedinE.coli.IthashighaffinitytoL-arginineandhydrolyzesL-argininetocitrullineandammonia.LowconcentrationsofADIhavebeenshowntoinhibitproliferationincertainculturedcellsbyarrestingthecellcycleinG1and/orS phase.HigherconcentrationsofADIleadtosubsequentapoptosis.RecombinantMycoplasmaArginineDeiminaseisa46.3 kDaproteinconsistingof409 amino acids.
AminoAcidSequence
SVFDSKFKGIHVYSEIGELESVLVHEPGREIDYITPARLDELLFSAILESHDARKEHKQFVAELKANDINVVELIDLVAETYDLASQEAKDKLIEEFLEDSEPVLSEEHKVVVRNFLKAKKTSRELVEIMMAGITKTDLGIEADHELIVDPMPNLYFTRDPFASVGNGVTIHYMRYKVRQRETLFSRFVFSNHPKLINTPWYYDPSLKLSIEGGDVFIYNNDTLVVGVSERTDLQTVTLLAKNIVANKECEFKRIVAINVPKWTNLMHLDTWLTMLDKDKFLYSPIANDVFKFWDYDLVNGGAEPQPVENGLPLEGLLQSIINKKPVLIPIAGEGASQMEIERETHFDGTNYLAIRPGVVIGYSRNEKTNAALEAAGIKVLPFHGNQLSLGMGNARCMSMPLSRKDVKW
Source
E.coli
Purity
97%
BiologicalActivity
MeasuredbyitsabilitytoinduceapoptosisinJurkatcellsusingaconcentrationof100–150 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C