Type
Recombinant
Description
Total102 AA.MW:11 kDa(calculated).UniProtKBacc.no.Q7TM98.N-TerminalHis-tag16 AA(highlighted).
AminoAcidSequence
MRGSHHHHHHGMASHMTLESIVEKKVKELLANRDDCPSTVTKTFSCTSITASGRLASCPSGMTVTGCACGYGCGSWDIRDGNTCHCQCSTMDWATARCCQLA
Source
E.coli
Purity
>95%
SDS-PAGEGel
12%SDS-PAGEseparationofMouseRELMgamma
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,10μg/lane
3.non-reducedandnon-heatedsample,10μg/lane
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05MAcetatebufferpH 4
Reconstitution
Add0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
Note
Thisproductisintendedforresearchuseonly.