Type
Recombinant
Description
Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsat,oradjacentto,specificresiduesoraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.Proteasesplayanimportantroleinmostdiseasesandbiologicalprocessesincludingprenatalandpostnataldevelopment,reproduction,signaltransduction,theimmuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,frequentlyusedintheanalysisandproductionofproteins.Carboxypeptidase-BsequentiallycleavesC-terminalK andRresidues.RecombinantRatCarboxypeptidase-Bisa35.1 kDaproteinconsistingof307 amino acids.
AminoAcidSequence
ASGHSYTKYNNWETIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKTRPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNIDGYVYTWTKDRMWRKTRSTMAGSSCLGVDPNRNFNAGWCEVGASRSPCSETYCGPAPESEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQIRQTCEETMLAVKYIANYVREHLY
Source
E.coli
Purity
95%
BiologicalActivity
Carboxypeptidase-BsequentiallycleavesCterminalK andRresidues.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C