Type
Recombinant
Description
SDF-1αandβarestromal-derived,CXCchemokinesthatsignalthroughtheCXCR4receptor.SDF-1αandβchemoattractBandTcells,andhavebeenshowntoinducemigrationofCD34+stemcells.Additionally,theSDF-1 proteinsexertHIV-suppressiveactivityincellsexpressingtheCXCR4receptor.HumanandmurineSDF-1 proteinsactacrossspecies.SDF-1αandβcontainthefourhighlyconservedcysteineresiduespresentinCXCchemokines.ThematureSDF-1αproteinistheresultofalternativesplicingoftheSDF-1 geneandcontains68 aminoacidresidues.RecombinantMurineSDF-1αisa7.9 kDaproteincontaining68 aminoacidresidues.
AminoAcidSequence
KPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK
Source
E.coli
Purity
98%
BiologicalActivity
Determinedbyitsabilitytochemoattracthumanperipheralbloodmonocytesusingaconcentrationof50–100 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C