Type
Active
Description
Total99 AA.MW:10.7 kDa(monomer),proteinactiveas dimer
AminoAcidSequence
PQFSLWKRPVVTAHIEGQPVEVLLDTGADDSIVAGIELGSNYSPKIVGGIGGFINTKEYKNVEIEVLNKRVRATIMTGDTPINIFGRNILASLGMSLNL
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis: >95%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanHIV-2 Protease
1. M.W.Marker –10,20,30,40,60,80 kDa
2.reducedandheatedsample,2.5 μg/lane
Formulation
20 mMTris,20 mMMES,200 mMNaCl,10%glycerol,1 mMEDTA,0.5 mMDTT,0.05%PEG8000,pH7.0 –filtered(0.4 μm), frozen
Reconstitution
Defrostatambienttemperature.
Applications
Kineticstudies,Inhibitorscreening,Crystallography
Shipping
Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.
QualityControlTest
SDSPAGEtodeterminepurityoftheprotein.
Activesitetitrationbytightlybindinginhibitor.
Note
Km=740 μMKcat=3 s-1 Kcat/Km=4.1 mM-1 s-1 withpeptidesubstrateATLNFPISPW