Type
Recombinant
Description
AllthreeisoformsofGROareCXCchemokinesthatcansignalthroughtheCXCR1orCXCR2receptors.TheGROproteinschemoattractandactivateneutrophilsandbasophils.RecombinantMurineKCisa7.8 kDaproteinconsistingof72 aminoacids,includingthe‘ELR’motifcommontotheCXCchemokinefamilythatbindstoCXCR1or CXCR2.
AminoAcidSequence
APIANELRCQCLQTMAGIHLKNIQSLKVLPSGPHCTQTEVIATLKNGREACLDPEAPLVQKIVQKMLKGVPK
Source
E.coli
Purity
98%
BiologicalActivity
Determinedbyitsabilitytochemoattracttotalhumanneutrophilpopulationusingaconcentrationrangeof10.0–100.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C