Type
Recombinant
Description
GM-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofneutrophilsandmacrophages,andpromotestheproliferationanddevelopmentofearlyerythroidmegakaryocyticandeosinophilicprogenitorcells.Itisproducedinbyendothelialcells,monocytes,fibroblastsandT-lymphocytes.GM-CSFinhibitsneutrophilmigrationandenhancesthefunctionalactivityofthematureend-cells.Thehumanandmurinemoleculesarespecies-specificandexhibitnocross-speciesreactivity.RecombinantRatGM-CSFisa14.5 kDaglobularproteinconsistingof128 aminoacidresidues.
AminoAcidSequence
MAPTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK
Source
E.coli
Purity
98%
BiologicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofMurineFDC-P1cellsis≤0.01 ng/ml,correspondingtoaspecificactivityof≥1.0×108units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C