Type
Recombinant
Description
SPARC/Osteonectinisasecreted,evolutionarily-conserved,collagen-bindingglycoproteinthatisinvolvedinavarietyofcellularactivities.Itishighlyexpressedintissuesundergoingmorphogenesis,remodelingandwoundrepair.SPARC/Osteonectinanditsrelatedpeptidesbindtonumerousproteinsoftheextracellularmatrix(ECM),affectECMproteinexpression,influencecellularadhesionandmigration,andmodulategrowthfactor-inducedcellproliferationandangiogenesis.SPARC/Osteonectinconsistsofthreedomains:anN-terminalacidicregionthatbindscalciumionswithlowaffinity,amodulecontainingtwoEF-handmotifsthatbindcalciumwithhighaffinity,andacysteine-richfollistatin-likedomain.RecombinantHumanSPARC/Osteonectinisaglycoproteincontaining286 aminoacidsthatmigratesatanapparentMWof43.7 kDabySDS-PAGEanalysisduetotheeffectofglycosylation.ThecalculatedmolecularweightofRecombinantHumanSPARC/Osteonectinis32.7 kDa.
AminoAcidSequence
APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
Source
ChineseHamsterOvaryCells(CHO)
Purity
98%
BiologicalActivity
DeterminedbyitsabilitytoincreasealkalinephosphataseactivityindifferentiatingMC3T3cellsusingaconcentrationof0.5–0.7 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C