Nordicmubio/Goat anti Chicken proteins/GACh/TSP

Background:

Inimmunoelectrophoresisagainstpooledserumandenrichedserumproteinsfractionsprecipitationcanbeobservedofnotlessthan11individualproteinscomponents.InprecipitatingtechniquesasimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony)toidentifytheserumproteinpattern,orthepresenceorabsenceofanindividualcomponent.Toevaluatethepurityofanisolatedserumproteinincludingimmunoglobulins.Sinceimmunoprecipitationdependsonacorrectantigen/antibodyconcentrationratio(zoneofequivalence)inthegelmedium,theproteinanalysisbyimmunoelectrophoresisofserumoranyotherBIOLOGicalfluidorproteinfractionshouldincludedifferentproportionsofthereactants.ItisnotpossIBLetoobtainanoptimalproteinpatterninasingleanalysis.Theelectroendosmosiseffectofdifferenttypesofagaronproteinswithadifferentnetchargecanbeusedtooptimizetheresolutionpowerofthetestsystem.AgarNordicNr.2containssufficientpositivelychargedionstooptimizetheresolutionoftheproteinsinthebeta-gammaregions,whilethealpharegionswillbecomemoredense.Highlypurifiedagar(AgarNordicnr.1)withlowelectroendosmosisfavourstheresolutionoftheproteinsinthealpharegions,whilethemajorcomponentsinthebeta-gammaregioncanstillbeidentified.

Source:

Pooledwholechickenserumandpartlypurifiedserumfractions.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Delipidated,heatinactivated,lyophilized,stablewholeantiserum.Nopreservativeadded.TotalproteinandIgGconcentrationsintheantiserumarecomparabletothoseofpoolednormalgoatserum.Noforeignproteinsadded.Reconstitutethelyophilizedantiserumbyadding1mlsteriledistilledwater.

Applications:

Precipitationassays.Inimmunoelectrophoresisuse2μlserumorequivalentagainst120μlantiserum.Indoubleradialimmunodiffusion(Ouchterlony)usearosettearrangementwith10μlantiserumin3mmdiametercenterwelland2μlserumsamples(neatandseriallydilutedin2mmdiameterperipheralwells.Differentbleedingsoftheimmunizedanimalsarepooledtoobtainabroadspectrumbalancedagainstthevaryingconcentrationsoftheindividualserumproteincomponents.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoanimalproteinssincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThisantiSerumhasnotbeenadsorbedforsuchcross-reactivity.Consequentlyitisnotspecies-specific.

Specificity:

PrecipitatingpolyclonalGoatantiSerumtoChickenSerumproteins

Storage:

Thelyophilizedantiserumisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Dilutionsmaybepreparedbyaddingphosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheantiserum.Dilutedantiserumshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.