Background:
ThereactivityoftheantiserumisdirectedtotheFcandFabsubunitsoftheIgGmolecule.ItincludessomereactivitywithotherimmunoglobulinsviathecommonFabportion.Itdoesnotreactwithanynon-Igproteininbovineserum,astestedbyimmunoelectrophoresisanddoubler
ADIalimmunodiffusion.ToidentifyandmeasureIgG,antigenorantibody,atthecellularandsubcellularlevelbyimmunofluorescencestainingofappropriatelytreatedcellandtissuesubstrates,andtodemonstratecirculatingantibodiesin
SEROdiagnosticmicro
BIOLOGyandautoimmunediseases;toidentifyaspecificantigenorimmunecomplexusingareferenceantibodyofbovineorigininthemiddlelayeroftheindirecttestprocedure.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.
Source:
PurifiednormalIgGisolatedfrompooledbovineserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.
Product:
FITC-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded.Itisreconstitutedbyadding2mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.
Applications:
ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence.
CrossReactivity:
Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail,howevertheconjugatehasbeentestedindoubleradialimmunodiffusion(Ouchterlony)antiHumanandSwineSerumandnoreactionhasbeenobserved.
Specificity:
Fluoresceinisothiocyanate-conjugatedIgGfractionofpolyclonalGoatantiSerumtoBovineIgG,heavyandlightchains
Storage:
Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.
Caution:
ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,but
Nordic-Mubioacceptsnoli
ABIlityforanyinaccuraciesoromissionsinthisinformation.