Nordicmubio/Beta-Defensin-2 (HBD-2/MBD-2)/DEF02-S

Product Type	Primary Antibodies
Units	100 µl
Host	Rabbit
Application	ELISARadioimmunoassay

BackgroundHuman defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. Originally defensins were isolated from hemolysates, but they can also be found on the cell surface of epithelia of the urogenital tract, lung, and trachea. In leukocytes defensins are stored in granules being released into phagolysosomes for the destruction of incorporated microorganisms. Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. ß-defensin-2 (HBD-2) whole molecule andN-terminal fragments of HBD-2, no cross-reactivity to alpha-defensin and other beta-defensins, full cross-reaction with mouse and rat beta-defensin-2.

Source

Immunogen: Mixture of synthetic peptides (14 AA near N-terminus of the mature HBD-2, 14 AA near N-terminus of the mature rat ß-Defensin-2, and 17 AA near N-terminus of the mature mouse ß-Defensin-2) coupled to KLH for immunisation of rabbits

ProductRabbit serum lyophilized with PBS and sodium azide 0.05%

Purification Method: Rabbit serum lyophilized with PBS and sodium azide 0.05%

Specificity

Species Reactivity: Human, mouse, rat, other species not tested

ApplicationsELISA, RIA, IHC not established

Working Concentration: (lyophilisate) 1:1000 - 1:5000 WB, 1:10000 - 1:50000 ELISA

Positive Control: Skin or oral mucosa

Storage-20'°C'

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Harder J., Bartels J., Christophers E., and J.-M. Schröder (1997) Antibacterial peptide specific for Gram-negative Bacteria / also effective for Candida albicans. Nature 387; 861ff 2. Hiratsuka T., Nakazato M., Date Y., Ashitani J., Minematsu T, Chino N., and S. Matsukura (1998) Biochem. Biophys. Res. Comm. (Pharmacol.) 249; 943 ff

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf