Pre-madelentiviralparticlesexpressingafusiontargetof(CFP-humanP53). Thesequenceverified humanP53gene codonsequencewasfusionedatitsN-terminalwithCFPprotein,expressedunderaconstitutivesuCMVpromoter, containingapuromycin antibiotic Markerunderthe RSVpromoter.
HumanP53(NCBIaccessionID:NM_000546)involvsinmanysignalpathway.ThefluorescentlabeledP53canbeusedto visualizesub-cellularpathwaywherethe P53 boundortargetedat.
Pleaseseeproductmanualfordetails.
Amount:200ul/vial(1×108IFU/ml), providedinDMEMmediumwith10%FBSand60ug/mlPolybrene(10x).
Cat#:LVP448-C