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Pre-made lentiviral particles bicistronically express two individual proteins: firefly luciferase and nuclear permeable CRE recombinase, under the same suCMV promoter. A GFP-Puromycin fusion dual marker was expressed under RSV promoter, which allow to select the positive transduced stable cells for long term culture via GFP cell sorting or via antibiotic selection. Both lucifease and CRE are highly active, best for both enzymes' assays In vivo or In vitro. CRE protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. CRE lentiviral particles can catalyze the jointing event In Vivo in the presence of lox sites. This nuclear permeant CRE (NLS-CRE) increases the recombination events in vivo. Purified CRE enzyme can joint individual plasmids containing lox sites. Luciferae and NLS-CRE are constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. Luciferase and GFP provide the convenient, realtime monitoring signals. This particles is concentrated and buffer changed into PBS solution, best suitable for hard-to-transduced cell lines, or serum-sensitive cell lines and in vivo application. Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS. Cat#: LVP412-PBS
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g