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Gentarget/CRE2ARFP (Bsd), EF1a lentivirus in PBS/LVP522PBS/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Gentarget/CRE2ARFP (Bsd), EF1a lentivirus in PBS/LVP522PBS/1 Ea

Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheRFPMarkerunderthesameenhancedEF1apromoter.CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibioticselection.

CREandRFPare constitutivelyexpressedinhighlevelindependentofcelltypes.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  

Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

ParticleswasconcentratedandresuspendedinPBSsolution,bestsuitableforserum-freeculture or hard-to-transducedcellline, ordireclyused forinvivoapplication.

Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at 5x107IFU/mlinPBS. 

Cat#:LVP522-PBS


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